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Ligation products were purified; reverse transcribed and amplified using adaptor primers.
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DNase I treatment with Qiagen RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
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HCC and adjacent normal tissues were prefrozen with liquid nitrogen, ground into powder with mortar, extracted with Trizol regeant according its protocal.

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Libraries were bead mill style block using glass beads can be considered in media to support in a bullet blender users.
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Rna was further using protocol requirement for rna using qiagen rneasy columns for sequencing was used for more economical bead based beads and embryos from streptomyces sp.
One nanogram of reference RNA was also amplified using the same procedure.
Bovine tails were isolated from freshly slaughtered cattle, the discs excised and macroscopically dissected into AF and NP tissues taking care to remove any transition zone.
RNAse free deionsed water and quantified using the Nanodrop spectrophotometer.
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Total RNA was isolated separately in different stages in late petal, early petal and in scape sample and same amount of total RNA was pooled before RNA amplification procedure.
The Comet assay, adapted from the protocol of Dr.
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Total RNA was isolated using a modified TRIZOL extraction method as follows.
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